| 1) OLa libarary |
| RNA source |
Male and female adult whole body of HNI inbred strain (male and female) |
| Vector |
pME18s （AB009864 1193,1194 modified from CC to AT) |
| adaptor sequence |
5' AGGAUCGAGUCGGCCUUGUUGGCCUAGUGG 3' |
| cloning site |
DraIII |
| Sequencing primer for 5' region |
CTT CTG CTC TAA AAG CTG CG |
| Host strain |
Top10 |
| produced by |
S.Sugano, K.Kawakami, M.Suzuki ( The Institute of Medical Science The University of Tokyo ) |
| clone request |
K.Naruse (naruse@biol.s.u-tokyo.ac.jp) |
| |
| 2) OLb libarary |
| RNA source |
Cultured medaka cell, OLHNI derived from caudal fin of adult male HNI inbred strain |
| Vector |
pBluescriptSK(+) |
| adaptor sequence |
oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' |
| cloning site |
EcoRI, SalI (disappeared after cloning) |
| Sequencing primer for 5' region |
TCG AAA TTA ACC CTC ACT AAA GGG |
| Host strain |
XL1 Blue MRF' |
| produced by |
H.Mitani |
| clone request |
K.Naruse (naruse@biol.s.u-tokyo.ac.jp) |
| |
| 3) OLc library |
| RNA source |
Cultured medaka cell, OLHNI derived from caudal fin of adult male HNI inbred strain (with 20J/m2 UV ± PR) |
| Vector |
pUC118 |
| adaptor sequence |
oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence 5'-OH-AATTCGGCACGAGG-3' |
| cloning site |
EcoRI, SalI (disappeared after cloning) |
| Sequencing primer for 5' region |
GGA AAC AGC TAT GAC CAT GAT TAC |
| Host strain |
E. coli SURE |
| produced by |
H.Mitani |
| clone request |
K.Naruse (naruse@biol.s.u-tokyo.ac.jp) |
| |
| 4) OLd library |
| RNA source |
Ovary of HNI inbred strain |
| Vector |
pUC118 |
| adaptor sequence |
oligo(dT)18 linker primer sequence (GA)10ACTAGTCTCGAG(T)18・adaptor sequence
5'-OH-AATTCGGCACGAGG-3' |
| cloning site |
EcoRI, SalI (disappeared after cloning) |
| Sequencing primer for 5' region |
GGA AAC AGC TAT GAC CAT GAT TAC |
| Host strain |
XL1 Blue MRF' |
| produced by |
H.Mitani, K.Naruse, M.Kondo |
| clone request |
K.Naruse (naruse@biol.s.u-tokyo.ac.jp) |
| |
| 5) OLe library |
| RNA source |
Liver of HNI inbred strain (male and female) |
| Vector |
pUC118 |
| adaptor sequence |
oligo(dT)18
linker primer sequence
(GA)10ACTAGTCTCGAG(T)18・adaptor sequence
5'-OH-AATTCGGCACGAGG-3' |
| cloning site |
EcoRI, SalI (disappeared after cloning) |
| Sequencing primer for 5' region |
GGA AAC AGC TAT GAC CAT GAT TAC |
| Host strain |
XL1 Blue MRF' |
| produced by |
H.Mitani, K.Naruse, M.Kondo |
| clone request |
K.Naruse
(naruse@biol.s.u-tokyo.ac.jp) |
| |
| 6) OLf library |
| RNA source |
Cultured medaka cell, OLHNI
derived from caudal fin of adult male HNI inbred strain (with
gamma-irradiation) |
| Vector |
pBluescriptSK(+) |
| adaptor sequence |
oligo(dT)18
linker primer sequence
(GA)10ACTAGTCTCGAG(T)18・adaptor sequence
5'-OH-AATTCGGCACGAGG-3' |
| cloning site |
EcoRI, SalI (disappeared after cloning) |
| Sequencing primer for 5'
region |
TCG AAA TTA ACC CTC ACT AAA GGG |
| Host strain |
E. coli SURE |
| produced by |
H.Mitani |
| clone request |
K.Naruse
(naruse@biol.s.u-tokyo.ac.jp) |
| |
| 7) SNK01 library |
| RNA source |
Eye ofWild population from Okayama Pref. (Southern part of Japan) |
| Vector |
pUC118 |
| Sequencing primer for 5' region |
GGA AAC AGC TAT GAC CAT GAT TAC |
| Host strain |
XL1 Blue MRF' |
| produced by |
E.Sanaka, H.Hori |
| clone request |
H.Hori (hori@biol1.bio.nagoya-u.ac.jp) |
| |
| 8) MF01SSA library |
| RNA source |
Whole embryo Develop. stage: segmentation stage 20 - 25 |
| Vector |
|
| Sequencing primer for 5' region |
|
| Host strain |
|
| produced by |
Kohara,Y., Shin-i,T., Kimura,T., Narita,T., Jindo,T., Takeda ,H. |
| clone request |
H.Takeda (htakeda@biol.s.u-tokyo.ac.jp) |
| |
| 9) CAB library |
| RNA source |
Embryonic stage 24 of inbred medaka CAB strain |
| Vector |
pSPORT (Invitrogen/Life Technologies/GibcoBRL) |
| Sequencing primer for 5' region |
T7: TAATACGACTCACTATAGGG |
| Host strain |
DH5 alpha |
| produced by |
J. Wittbrodt, B. Wittbrodt, C. Burgtorf
clone request J. Wittbrodt (Jochen.Wittbrodt@EMBL-Heidelberg.de)
|
| clone request |
|